特邀学术报告（两场）

Title:Metabolomics as a Tool in Dereplication studies of Microbial symbionts

Lecturer: Dr. RuAngelie Edrada-Ebel

Time: 2011年5月30日(星期一) 上午9:30

ADDRESS: 过程大厦312

Abstract of the lecture

Metabolomic methods can also be utilised to screen diverse biological sources of potentially novel and sustainable sources of antibiotics and pharmacological-active drugs. Dereplication studies by LC-HRFTMS and NMR can establish the chemical profile of endophytic and/or endozoic microbial extracts and their plant or animal sources. Identifying the compounds of interest at an early stage will aid in the isolation of the bioactive components.

Metabolomics is being applied to identify and biotechnologically optimize the production of pharmacologically active secondary metabolites. The links between metabolome evolution during optimisation and processing factors can be identified through metabolomics. Information obtained from a metabolomics data can efficiently establish cultivation and production processes at a small scale which will be finally scaled up to a fermenter system, whilst maintaining or enhancing synthesis of the desired compounds. MZmine and SIEVE softwares are utilized to perform differential analysis of sample populations to find significant expressed features of complex biomarkers between parameter variables. Metabolomes are identified with the aid of existing high resolution MS and NMR records from online or inhouse databases like AntiMarin, a database of microbial secondary metabolites and marine natural products. This is further validated through available reference standards and NMR experiments. Metabolomics has become a powerful tool in systems biology which allows us to gain insights into the potential of natural marine isolates for synthesis of significant quantities of promising new agents, and allows us to manipulate the environment within fermentation systems in a rational manner to select a desired metabolome. 

Personal Introduction

Dr. RuAngelie Edrada-Ebel is a lecturer in SIPBS, Strathclyde. She has a PhD in Pharmaceutical Biology University of Würzburg Germany, & did a postdoctoral fellowship at the University of California, Santa Cruz. In 2001, she accepted a researcher position at the University of Düsseldorf then joined the University of Strathclyde in December 2008. She is author of more than 80 publications & two patents. Her expertise comprises both natural product isolation & structure elucidation with modern spectroscopic techniques. At present, she has a series of projects on the application of metabolomics to identify & optimize the production of bioactive secondary metabolites in marine microorganisms. She is a member of the editorial Board for Marine Drugs. She contributes to part of the metagenomic project of the Beaufort Biodiscovery Award for a Marine Biodiscovery Programme.

Title:Real time Modelling in Bioprocesses

Lecturer: Dr. Mariana Fazenda

Time: 2011年5月30日(星期一) 上午9:30

ADDRESS: 过程大厦312

Abstract of the lecture

Biopharmaceuticals, such as herceptin (trastumazab) which is used in breast cancer treatment, have revolutionised the treatment of many serious diseases. But our understanding of how the interaction of the genetic alterations we introduce, and the bioreactor environment we culture the cells within, impact upon the cell metabolism is quite limited, especially in early development phase. Our approach is to focus on cutting edge techniques upon achieving better understanding of the behaviour of these expression systems early in the development phase. The use of in-situ, non-invasive monitoring techniques (near infrared spectroscopy) together with a previously non real time metabolic analysis tool (flux balancing) to gain real time understanding of the metabolism of these specialised cells when in culture is shown. These will be deployed to monitor metabolite fluxes in continuous cultures of a major industrial expression system for biopharmaceuticals, Pichia pastoris. Finally, the predictive capability of the formulated real time steady state flux models will be examined in fed-batch and batch systems in terms of their ability to manipulate cell metabolism in a real time environment. This technology would give increased knowledge of cell metabolism early in the process cycle, helping accelerate product development, leading to reduced cost therapeutics, reaching patients more speedily. This approach could also help the development of specialised cell based therapies (e.g. stem cells). This is especially important as these agents are even more complex than biopharmaceuticals, and have tremendous potential to contribute to enhancing the health and welfare of society in the immediate future.

Personal Introduction

DrFazenda is a postdoctoral research fellow at the Fermentation Centre at Strathclyde. She has a degree in Biological Engineering from Technical University of Lisbon(2003); a PhD from Strathclyde with Masterfoods where process monitoring techniques e.g.NIRS & multivariate data analysis were applied to production of a novel protein. Further industrial experience at Millipore, & her involvement with GSK in cell culture technology broadened her expertise in Industrial Process Analysis through the use of PAT tools for the development of biopharmaceuticals in the UK. She recently published 2 reviews & 2 papers(15,16) on both fungal physiology & NIRS. She participated in the competition Biotechnology Young Entrepreneurs Scheme, UK (2009) & presented at the International Forum of Process Analytical Technology (IFPAC) in Baltimore, 2010